pQCasTns(Ptr)-array 8

LOCUS       pQCasTns(Ptr)-array 8               11767 bp ds-DNA     circular SYN 26-OCT-2021
DEFINITION  Expression of CRISPR-associated transposases.
ACCESSION   .
VERSION     .
KEYWORDS    pQCasTns(Ptr)-array 8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Yang S, Zhang Y, Xu J, Zhang J, Zhang J, Yang J, Jiang Y, Yang S
  TITLE     Orthogonal CRISPR-associated transposases for parallel and 
            multiplexed chromosomal integration.
  JOURNAL   Nucleic Acids Res. 2021 Sep 3. pii: 6363765. doi: 
            10.1093/nar/gkab752.
  PUBMED    34478496
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(316..939)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     847..866
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        958..1013
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    964..982
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    994..1012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(9965..9983)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9979..10026
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10256..11071)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10979..10998
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(11072..11163)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11181..49)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(11326..11345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgatcgtaa aaggatctca agaagatcct ttacggattc ccgacaccat cactctagat
       61 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
      121 gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
      181 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
      241 ttgcgctgat gaatccccta atgattttta tcaaaatcat taaggttacc atcacggaaa
      301 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
      361 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      421 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      481 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      541 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      601 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      661 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      721 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      781 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      841 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      901 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      961 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     1021 ctctagaaat aattttgttt aactttaaaa ggagatatac catgggtgaa ctgccgagta
     1081 ggcagctgga aatagaagcc atcgattaca aacgttgaac gactggtgaa ctgccgagta
     1141 ggcagctgaa gttagttgca tcagctgcgt gagctgttta tcgcggtgaa ctgccgagta
     1201 ggcagctgga aatgagatgg atgagtacat ccgtaaccac taatcgtgaa ctgccgagta
     1261 ggcagctgaa gttgcagcag taattcacgg ccaaaacgcc ggagagtgaa ctgccgagta
     1321 ggcagctgga aattgtctgc cgataacgcg ctttcattag tgccagtgaa ctgccgagta
     1381 ggcagctgaa gttacgcgga taaaatttaa tattctactc tggaagtgaa ctgccgagta
     1441 ggcagctgga aatgaggatg aagctaccgc gtaaagacag gttgcgtgaa ctgccgagta
     1501 ggcagctgaa gttggagtgt aggcctgata agacgctatc agcgtgtgaa ctgccgagta
     1561 ggcagctgga aatgtttaaa ctagggataa cagggtaatg gatccgaatt cgagctcggg
     1621 aagaaggaga tatacatatg cactttctgg tgcagaccaa gagctacccg gacgaggcgc
     1681 tggaaagcta tctgctgcgt ctggcgcgtg ataacagcta caacggttat agcgagctgg
     1741 cggacatcct gtggcagtgg ctggcggaac aagataacga gctggaaggt gcgctgccgc
     1801 tggcgctgag caaggtggac gtttaccacg cgcgtcaggc gagcagcttc cgtatccgtg
     1861 cgctgaaact ggtggcgcaa ctggcggacg ttaacgcggg tgatattctg gcgctggcgt
     1921 ggcgtcgtag caacttcaag tttggcaacc tggcggcggt gagccgtaac gagctggcga
     1981 tcccgctgga actgctgcgt accgataaca tcccggtttg cattaaatgc ctgagcgaga
     2041 gcagccacat tccgttttac tggcacctga agccgtataa agcgtgccac aagcacaaaa
     2101 gccagctgat cacccgttgc aaggagtgct acgacctgat tgattatcgt gcgagcgagg
     2161 cgtttctgga atgcgtttgc ggttgcaaaa tcaccaacag cgaacaactg aacgacgcgg
     2221 atttcaagat cgcgattgcg ctggcgagca gcaacagcca gaaaatcgtg ggcctgatta
     2281 gctggttcgc gaaggtgaaa caactggacg ttagcgacgc ggatttcaac tgcgcgtttg
     2341 ttgattactt caacacctgg ccggagagcc tgaccaccga actggacctg ctgaccaaca
     2401 acgcgcgtct gaagcagctg aacccgttta acaagaccaa attcagcagc gtgtacggtg
     2461 acctgatccg tgatggccaa attgcggcga ccagcaaccg taagaacaaa gttatcgacg
     2521 agatcattag ctattttgtg gaactggttg atagcaaccc gaaggcgaaa cacccgaaca
     2581 ttggtgacct gctgctgtgc accttcgatg cggcggtgct gctgaacacc accaccgagc
     2641 aggtttaccg tctgcaccaa gaagcgtttc tgaactgcgc gtatagccag aagaaacacg
     2701 aacaactgcg tgcggatagc cacgtgttct atctgcgtca ggttatcgag ctgcagcaag
     2761 cgtttgcggc ggaaaaaccg ctgaccaaga aacaattcat tgcgccgtgg taacttatga
     2821 acctgcagga tgcgctggcg attgagccgc tgaaggaaaa aaccaccgcg ctgcgtaagc
     2881 tgttcgtgcc gtacaccagc cacgttgagg tggatggttt tgaggaactg gcgctgaccg
     2941 tgctgatcaa cctggtttat aagcgtagcg aaattgacga tctgaccagc gcgcgtaccg
     3001 cgaaaagcgt gctgcgtgac gaggttctgc tgagcaagtg catcaacgaa gtgaaatggt
     3061 tccacaccca caacctgaag tacccggaca tccgtgttag ccaccaacgt ctgattagcg
     3121 aggtggttag cgaagatatc gcgggtattt gcagccgtag cctgccgctg agctttggct
     3181 ggagccacaa cagcgcggag atcaaccacg cgaaactgtt cctgaccagc tttaactggc
     3241 agggtgaagt gacctgcctg gcgcgtctgc tgattaacga ggaaccggtt tggatcaacc
     3301 tgattcgtgc gtacggtttc accaagaaag cggttctgga gatcagcggc aagattaaac
     3361 agcaactgcc ggtggcggag ttcccgctgg aagttagcag ctttagcccg cagctgcaaa
     3421 tgccgtttca gcaaagctat ctggtggtta ccccggtggt tagccacgcg atgctggcga
     3481 agatccagca actgaccacc gaccgtaaac tgaacttcgc gctggttgag cacagccgtc
     3541 cggcgaacgt tggtgatctg gcgagcagcg tgggtggcaa cattcgtgtt ctgcgttact
     3601 ttccgaagac ctatagcaaa gcggtgaacc gtagcaaagt tgcgaacaac gatatcgaaa
     3661 aggcgttcaa aattcgtgcg ctgctgagca gccagtttca gcaagcgctg ctggtgctgg
     3721 ttggcatcaa gcagttcaac accctgcgtc aaaaacgtct ggcgcgtgtg gcggcgatcc
     3781 gtcaagtgcg tgttagcctg caactgtggc tggacaacat tctggaggcg aagaacaacg
     3841 cgcagaacca agtgtacccg gaatgggttc gtcactatct ggatcaaagc atcaccaact
     3901 gcattagcca gttcagcaac gttctgaacg aaagcctggg taacctgagc aagctgaaac
     3961 gttttgcgta ccacccgaac ctgatgggcc tgttcaaagc gcaactgaac tatgtgttta
     4021 cccactgcgc ggcggagcag gaaatcctga acgacgagca aattgtgtac gttcactgcc
     4081 aggacatgcg tgttttcgat gcggaagcga tggcgaaccc gtatatccag ggtatgccga
     4141 gcctgaccgc gctgaacggc ctggcgcaca acttcgagcg taagctgaaa aactttattg
     4201 atccgagcat caagtgcatt ggtagcgcga tctacattga gaactatcaa ctgcacaccg
     4261 gcaaaccgct gccggaaccg agcaagctga aacaggtggc gggtcgtagc cacgttatcc
     4321 gtagcggcat cattgacaag ccgaaatgcg acattaccct ggatctggtg ttccgtctgt
     4381 ttgttccgaa caccgaactg ctggataagc tgaacagcca actgattaag ccggcgctgc
     4441 cgagcagctt tgcgggtggc accatgcacc cgccgagcct gtaccagaac attgactggt
     4501 gccacgtgca caccaagccg agcgagctgt ttaagaaact gaaggcgaaa agcagcaacg
     4561 gtagctggct gtatccgagc aagaaagtgg ttaaaagctt cgaacagctg atcgacgcgc
     4621 tgaacagcaa ctttaacctg cgtccggcgg cgattggcct ggcggcgctg gaggaaccgg
     4681 tgaagcgtga tgcggcgctg cacgagtacc actgctatgc ggaaccggtt atcggtctgc
     4741 tggagtgcgt gagcaacacc agcgttaagt acgcgggcgc gaaacaattc tttcacgacg
     4801 cgttctgggt gatggatgtt cagaaggaaa gcatgctgat gaagaaaagc aaatttgagt
     4861 atgaataatg cagctgccgc gtcacctgag ctacacccgt agcctgagcc cgagcaaggc
     4921 ggtgttcttt tataaaaccc cggagagcga cttcgaaccg ctgcagatcg agcaaaacaa
     4981 actggtgggt cagaagagcg gttttggcga tgcgtaccag aagcaaaacg ttgcgaaaaa
     5041 cctggcgccg caggacctgg cgtttggtaa cccgcaaacc attgatgtgt gctatgttcc
     5101 gccgaccgtg aacgaactgt tctgccgttt tagcctgcgt gttgaggcga actgcatcga
     5161 accgcacgtg tgcgacgatc cgaaggttat ttactggctg aaacgtttct ttgaaaccta
     5221 taagaaacac aacggtctga acgaagtggc gacccgttac gcgaagaaca tcctgatggg
     5281 caactggctg tggcgtaacc gtcagagccc gaacgttgac atcgagattc tgaccgaaca
     5341 cgcggcgccg attgtggttg agggtgcgca gaagctgaaa tggcaaggca actggcagaa
     5401 caaccaaacc gcgctgctga ccctgagcga gagcatccag gaaggtctga gcaacccgca
     5461 aaactactgc tatctggata tcaccgcgaa gattaaaaac gcgttcagcc aggaagtgca
     5521 cccgagccaa aagtttgtgg acaacgttga acagggtatg agcagcaaac agctggcgta
     5581 tacccaagtg ggcgataaga aagcggcgag cctgaacagc cagaaggttg gcgcggcgat
     5641 ccaaaccatt gacgattggt acgaggaagg ttataaaccg ctgcgtaccc atgagtatgg
     5701 tgcggacaag caaatcctgg tggcgcaccg taccccgaaa agccacagcg atttttatag
     5761 cctgctgccg cgtatcgcgc tgcacattaa gcacatggaa aaacacggtc tggagcagag
     5821 cgaacaaagc aacagcatcc acttcattgc ggcggttctg attaagggtg gcctgtttca
     5881 gcgtagcaaa ggatgaagcg ttactatttc accatcacct acctgccgca aagctgcgat
     5941 gtgagcctgc tggcgggtcg ttgcatcggc attctgcacg gtttcatgag cagccgtgag
     6001 atcagcaaca ttggcgtgtg ctttccgaaa tggaacgagc agaccatcgg taacgaactg
     6061 gcgtttgtta gcaccaacaa gaaacaactg accaacctga gccagcaaag ctatttcgag
     6121 atgatggcgc acgacaagct gtttggcctg agcaaaattc tggaagtgcc ggttaaccag
     6181 agcgaagtga tgttcgttcg taaccaaagc gtggcgaagg cgtttgttgg tgaaaagcaa
     6241 cgtcgtctga aacgtgcgaa gaaacgtgcg gaggcgcgtg gcgaagtgta caacccggag
     6301 tataagttcg aagcgaaaga tatcggtcac tttcacagca ttccggtgag cagcaagggt
     6361 aacggccaga gctacgttct gcacatccaa aagaacgaga acgcggaaag cattaaaaac
     6421 cagttcaaca actatggctt tgcgaccaac caaattttcc tgggcaccgt gccgagcctg
     6481 aacaccctgc tgtaaaagga gatataccat gtaccgtcgt aagctgaaat atagccgtgt
     6541 taagaacctg cacaaatttg cgagccagaa gaacaaaagc acctgcctgg tggagagcag
     6601 cctggaattc gacgcgtgct tccactttga gttcagcccg ccgatcgcgg cgtttgaagc
     6661 gcaaccgctg ggttacgagt atgaattcga taaccgtatt tgccgttaca ccccggactt
     6721 tctgctgacc cacaccgatg gcacccagaa gttcatcgag gttaagccgc aaagcaaaat
     6781 tgcggacgag gattttcgtg cgcgtttcat cgaaaagcag gcgattgcga aacaagacgg
     6841 tcgtgatctg atcctggtga ccgacaagca gattcgtgtt tacccgaccc tgaacaacct
     6901 gaaactgctg caccgttata gcggctttca gagcctgacc gagctgcaag cgagcgtgct
     6961 ggaactggtt aagcagtacg gtagcatcaa agtgggccaa ctgattcgtt atctgaaagt
     7021 taccgcgggt gaactgctgg cgaccgtgct gcgtctgctg agcctgggcc aactgttcgc
     7081 ggatctgacc accaacgaga tcagcattga aaccgcgatc tggagcaaca atgtttaata
     7141 acgacctgtt cgacgatgag tttaaccagc cgctgccgaa ggcggaaacc aaactgccgc
     7201 agaactatac caaggatctg caagcgctgc cggagaagat caaaaccacc accttcgcga
     7261 agctgaaata cattcaatgg ctggaggcga acatccaggg tggctggacc caaaagaacc
     7321 tggaaccgct gctgaaactg atgccggacg ttgagggtga aaagaaaccg agctggcgta
     7381 ccgcggcgcg ttggtatagc gcgtacacca acgcggataa gaacattatg gcgctgatcc
     7441 cgagccacca gaagaaaggc aaccgtgaac gtgacaccac caccgataag ttctttgaga
     7501 aagcgctgga acgttacctg gtgaaggaga aaccgagcgt tgcgagcgcg tataagttct
     7561 acaaagacct ggtgatcatt gaaaacgaca gcgtggttga tagcgttctg aaaccgctga
     7621 cctataaggc gtttaaaaac cgtattgaca acctgccgca gtatgaggtt atgatcgcgc
     7681 gttacggcaa gcgtctggcg gatattgcgt acaacaaggt ggaaggccac aaacgtccga
     7741 ttcgtgtgct ggagaaagtt gaaatcgacc acaccccgct ggatctgatt ctgctggacg
     7801 atgagctgca catcccgctg ggtcgtccga ccctgaccat gctggttgac gtttatagcc
     7861 actgcatcgt gggctactat ttcagcttta gcgagccgag ctacgatgcg gttcgtcgtg
     7921 cgatgctgaa cgcgatgaag ccgaaaagcg aagtggcgaa actgtacccg gacaccatta
     7981 acgagtggaa gtgcgcgggt aaaatcgaaa ccctggtggt tgataacggc gcggagttct
     8041 ggagcaacag cctggaactg gcgtgcgagg aaatcggtat taacacccag tataacccgg
     8101 tggcgaagcc gtggctgaaa ccgttcgttg agcgtatgtt tggcaccatc aacaccgaac
     8161 tgctggaccc ggttccgggc aagaccttca gcaacatcct gcaaaaacac gaatacaacc
     8221 cgaagaaaga cgcgattatg cgtttcacca cctttatgca gctgtttcac aagtgggtgg
     8281 ttgatgtgta tcaccaagac gcggatagcc gtttcaaata cattccgagc cagctgtggg
     8341 accaaggctt taacaccctg ccgccgacca tgctgagcga tgcggatctg cagcaactgg
     8401 atgtggttct gagcatcagc aaccaccgtg tgctgcgtaa gggtggcatt cgtctggaga
     8461 acctgagcta tgacagcacc gaactggcga actaccgtaa gcagttcagc cacaaagtga
     8521 gccaagaggt tctgatcaaa ctgaacccgg acgatattag ctacatctat gtgtacctgg
     8581 acaagctgga acactatatt aaagttccgt gcatcgatcc gaacggttac acccagaacc
     8641 tgagcctgaa ccaacacaag atcaacattc gtatccaccg tgactttatt agcggtagca
     8701 tcgataacgt tggcctggcg aaggcgcgta tgttcattca caacaaaatc cagaacgagt
     8761 ttgaggaact gaagaacgcg ccgaaacaca gcaaggtgaa aggtggcaag gcgctggcga
     8821 aacaccagaa cattagcagc gacagccaaa agagcatcac ccacagcaaa ccggtggagg
     8881 cgaagaaagt taccccgaaa gaacaaccga ccgatagctg ggacgatttc atcagcgacc
     8941 tggatggttt ttaattatgc tgaccgacaa gcagaaagaa aagctgaacg agttccgtga
     9001 tgtttttatt gaatacccga tcattaccac catcttcaac gactttgatc gtctgcgtct
     9061 gggtaaaggc ctgaccggcg agaagccgtg catgctgctg aacggtgaca ccggcaccgg
     9121 taaaaccgcg ctgattaaac agtataagga acgtcacctg ccgcaattca tcaacggtgt
     9181 tatgaaccac ccggtgctgg ttagccgtat tccgagcaac ccgaccctgg aaagcaccct
     9241 ggcggagctg ctgaaagacc tgggtcaagt gggcagcacc gagcgtaagc tgcgtattaa
     9301 cggcacccgt ctgaccacca gcctgatcaa atgcctgaag acctgcggca ccgaactgat
     9361 cattatcgat gagtttcagg aactgattga gcacaaccaa ggcaagaaac gtcgtgaaat
     9421 tgcgaaccgt ctgaaataca tcaacgacga ggcgggtgtt agcattgtgc tggttggcat
     9481 gccgtgggcg gaaaagatcg cggatgagcc gcagtggagc agccgtctgc tgatccgtcg
     9541 tcaactgccg tatttcaaac tgagcgagaa cccgaagcac tttgtgcagc tgattatcgg
     9601 tctggcgaac cgtatgccgt tcgcggaaaa accgaacctg agcgagcaag cgaccgtttt
     9661 caccctgttt agcctgagca aaggctgctt ccgtaccctg aagtactttc tggacgatgc
     9721 ggtgctgtat gcgctgatgg acaacgcgaa gaccctgacc accaaacacc tggtgaaggc
     9781 gttcgaagtt ctgtttccgg atgtgccgaa cctgtttacc ctgccggttg cggagatcac
     9841 cgcgagcgag gtggaacgtt acagcctgta taagccggaa agcagccagg acgaggaccc
     9901 gttcattgcg accaaattta ccgatcgtat gccgatcagc caactgctgc gtaagtaact
     9961 cgagccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
    10021 tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
    10081 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg
    10141 acaagctgac gaccgggtct ccgcaagtgg cacttttcgg ggaaatgtgc gcggaacccc
    10201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
    10261 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
    10321 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
    10381 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
    10441 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
    10501 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
    10561 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
    10621 gagcgagacg aaatacgcgg tcgctgttaa aaggacaatt acaaacagga atcgaatgca
    10681 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
    10741 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
    10801 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
    10861 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
    10921 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
    10981 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
    11041 agcaagacgt ttcccgttga atatggctca tactcttcct ttttcaatat tattgaagca
    11101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11161 aaataggcat gctagcgcag aaacgtccta gaagatgcca ggaggatact tagcagagag
    11221 acaataaggc cggagcgaag ccgtttttcc ataggctccg cccccctgac gaacatcacg
    11281 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
    11341 ttccccctga tggctccctc ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg
    11401 gtggaggctt tacccaaatc accacgtccc gttccgtgta gacagttcgc tccaagctgg
    11461 gctgtgtgca agaacccccc gttcagcccg actgctgcgc cttatccggt aactatcatc
    11521 ttgagtccaa cccggaaaga cacgacaaaa cgccactggc agcagccatt ggtaactgag
    11581 aattagtgga tttagatatc gagagtcttg aagtggtggc ctaacagagg ctacactgaa
    11641 aggacagtat ttggtatctg cgctccacta aagccagtta ccaggttaag cagttcccca
    11701 actgacttaa ccttcgatca aaccgcctcc ccaggcggtt ttttcgttta cagagcagga
    11761 gattacg
//